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KMID : 1123920170310050264
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Korean Journal of Oriental Physiology and Pathology
2017 Volume.31 No. 5 p.264 ~ p.369
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Protective Effects of Prunella Vulgaris on Glomerular I njury in Streptozotocin-I nduced Diabetic Rats
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Yoon Jung-Joo
Park Ji-Hun
Jeong Da-Hye
Han Byung-Hyuk
Choi Eun-Sik
Lee Yun-Jung
Kang Dae-Gill
Lee Ho-Sub
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Abstract
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Prunella vulgaris, well-known traditional medicinal plant, is used for the cure of abscess, scrofula, hypertension and urinary diseases. Diabetic nephropathy is the most common cause of end-stage renal disease. The pathological characteristics of diabetic nephropathy are glomerular and tubular basement membrane thickening. The aim of the present study was to evaluate the effect of Prunella vulgaris, on diabetic glomerular injury in streptozotocin ?induced diabetes rats. Diabetes mellitus was induced by a single intraperitoneal injection of streptozotocin (STZ; 45 mg/kg) and confirmed by random glucose level higher than ≤ 300 mg/dL. The experimental rats were divided into five groups: control group (Male SD rats), STZ group (Male SD rats injected STZ), Aminoguanidine group (Male SD rats injected STZ + AG 100 mg/kg/day), Low dose group (Male SD rats injected STZ + APV 100 mg/kg/day), High dose group (Male SD rats injected STZ + APV 300 mg/kg/day). AG or APVs were administered once a day for 8 weeks. Body weight and food/water intake were measured every four weeks. At the end of study, the kidneys were collected and cut into pieces for immunohistochemistry and western blot analysis. Our study showed that body weight and water/food intake were no significant differences between untreated STZ-induced diabetic rat and APV treated-STZ rat. However, phosphorylation of receptor-regulated Smads (Smad3) was significantly decreased in APV treated-STZ rat as compared with the diabetic group. In addition, APV was improved nephrin level in kidney tissue. Therefore, we suggest that APV has a protective effect against STZ-induced diabetic glomerular injury.
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KEYWORD
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Prunella vulgaris, Streptozotocin, Diabetic, Glomerular injury, Nephrin
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